Chapter 10 The Application of Labeled Lectins and Antibodies to Sds Gels And Their use In Studying Glycoproteins and Cell Surface Antigens During Development

摘要: Publisher Summary An approach has been developed by several laboratories for detecting both glycoproteins and antigens in sodium dodecyl sulfate (SDS) polyacrylamide gels the direct binding of lectins or antibodies, respectively. The is detected labeling lectin antibody with an enzymatic, fluorescent, radioactive marker. Because a characteristic developing systems expression new surface antigens, many which will also be glycoproteins, techniques (lectin staining gels) particular value analyzing changes during differentiation. chapter illustrates this using applied to analyze two differentiating systems: (1) development cellular slime mold, Dictyostelium discoideum (2) differentiation muscle cell line tissue culture. Lectin SDS provides sensitive technique according their apparent molecular weight carbohydrate content. resolution essentially equal gel system used. When used it suggests way purifying any interest becasue can affinity chromatography.