Phytochemicals and Cytotoxicity of Quercus infectoria Ethyl Acetate Extracts on Human Cancer Cells.

摘要: Conventional and modern cancer treatment were reported to manifest adverse effects the patients. More researches conducted search for selective cytotoxic agent of plant natural product on cells. The presences wide range phytochemicals in Quercus infectoria (QI) extract have been implicated with effect against various types cell which remain undiscovered. This present study aimed evaluate QI extracts selected human cells then, most potent was further analysed general phytochemical constituents. galls extracted successively n-hexane, ethyl acetate methanol yielded three main extracts; n-hexane (QIH), (QIEA) (QIM), respectively. qualitatively tannin, alkaloids, glycosides, saponins, terpenoids, flavonoids phenolic compounds. Next, tested determine activity cervical (HeLa), breast (MDA-MB-231) liver (Hep G2) using MTT assay. Cytotoxic normal fibroblast (L929) line also evaluated cytoselective property. Meanwhile, DMSO-treated served as negative control while cisplatin-treated positive control. then chosen be investigated DNA fragmentation hallmark apoptosis Hoechst staining. Qualitative analysis revealed presence QIEA exhibited HeLa (IC50 value = 6.33 ± 0.33 μg/mL) showed property L929 induced treated richness constituents might contribute potency towards