DMSP Lyase in Marine Macro- and Microalgae

作者: Michael Steinke , Claudia Daniel , Gunter O. Kirst

DOI: 10.1007/978-1-4613-0377-0_27

关键词:

摘要: The enzymatic cleavage of dimethylsulfoniopropionate (DMSP) to dimethylsulfide (DMS) was investigated in twenty-one strains marine macro- and microalgae, representing seven algal classes. enzymes involved this are DMSP lyases, producing DMS from DMSP. All tested were able synthesize accumulate various levels intracellular but only twelve showed lyase activity. It possible identify subgroups strong weak producers. first subgroup included three Enteromorpha species (E. clathrata, E. intestinalis, compressa) Phaeocystis sp. with specific activities crude cell extracts ranging 7 over 100 nmol min-1 (mg protein)-1. second composed a sub-antarctic strain Acrosiphonia arcta, Polysiphonia lanosa, two Emiliania huxleyi, sonderi, Ulva lactuca bulbosa. In activity ranged 0.01 0.2 No detectable sub-arctic Monostroma arcticum, Prasiola crispa, urceolata, Ascoseira mirabilis, Laminaria saccharina Tetraselmis subcordiformis. Non-optimal assay conditions bacterial contamination may have affected rates some samples, the results suggest widespread presence among taxa, also raises possibility that closely-related quite different or function.

参考文章(27)
M. D. Keller, Dimethylsulfide production in marine phytoplankton. Biogenic sulfur in the environment.. pp. 167- 182 ,(1989)
Biogenic sulfur in the environment ACS Symposium series (USA). ,(1989) , 10.1021/BK-1989-0393
G.L. Cantoni, David G. Anderson, E. Rosenthal, Enzymatic cleavage of dimethylpropiothetin by Polysiphonia lanosa. Journal of Biological Chemistry. ,vol. 222, pp. 171- 177 ,(1956) , 10.1016/S0021-9258(19)50782-7
U. Karsten, C. Wiencke, G. O. Kirst, The ß-dimethylsulphoniopropionate (DMSP) Content of Macroalgae from Antarctica and Southern Chile Botanica Marina. ,vol. 33, pp. 143- 146 ,(1990) , 10.1515/BOTM.1990.33.2.143
PT Visscher, MR Diaz, BF Taylor, Enumeration of bacteria which cleave or demethylate dimethylsulfoniopropionate in the Caribbean Sea Marine Ecology Progress Series. ,vol. 89, pp. 293- 296 ,(1992) , 10.3354/MEPS089293
J Stefels, WHM van Boekel, PRODUCTION OF DMS FROM DISSOLVED DMSP IN AXENIC CULTURES OF THE MARINE-PHYTOPLANKTON SPECIES PHAEOCYSTIS SP Marine Ecology Progress Series. ,vol. 97, pp. 11- 18 ,(1993) , 10.3354/MEPS097011
Maureen D. Keller, Rhonda C. Selvin, Wolfgang Claus, Robert R. L. Guillard, MEDIA FOR THE CULTURE OF OCEANIC ULTRAPHYTOPLANKTON Journal of Phycology. ,vol. 23, pp. 633- 638 ,(2007) , 10.1111/J.1529-8817.1987.TB04217.X
F. Larher, J. Hamelin, G.R. Stewart, L'acide diméthylsulfonium-3 propanoïque de Spartina anglica Phytochemistry. ,vol. 16, pp. 2019- 2020 ,(1977) , 10.1016/0031-9422(77)80117-9
F. M. M. Morel, J. G. Rueter, Donald M. Anderson, R. R. L. Guillard, AQUIL: A CHEMICALLY DEFINED PHYTOPLANKTON CULTURE MEDIUM FOR TRACE METAL STUDIES12 Journal of Phycology. ,vol. 15, pp. 135- 141 ,(1979) , 10.1111/J.1529-8817.1979.TB02976.X