Flow cytometry detection of vitamin D receptor changes during vitamin D treatment in Crohn's disease
作者： M. Bendix , A. Dige , B. Deleuran , J. F. Dahlerup , S. P. Jørgensen
DOI: 10.1111/CEI.12613
关键词:
摘要: Crohn's disease (CD) is a chronic inflammatory associated with dysregulated T cell response towards intestinal microflora. Vitamin D has immune modulatory effects on cells through the nuclear vitamin receptor (VDR) in vitro. It unclear how oral treatment affects VDR expression. The aim of this study was to establish flow cytometry protocol, including and cytoplasmic expression, investigate expression CD patients. protocol for staining developed using human acute monocytic leukaemia line (THP-1). evaluated anti-CD3/CD28-stimulated peripheral blood mononuclear (PBMCs) from D3- (n = 9) placebo-treated Anti-VDR-stained PBMCs were examined by cytometry, their cytokine production determined bead array. VDR, CYP27B1 RXRα mRNA levels CD4(+) measured quantitative reverse transcriptase polymerase chain reaction. enabled detection results confirmed confocal microscopy supported correlation increased following stimulation. This up-regulation inhibited 30% compared placebo patients (P 0027). correlated in-vitro interferon-γ stimulated 0.01). Flow useful method which measure intracellular reduces receptor-mediated up-regulation.
sci-hub.se 参考文章(38)
Anna Klopot, Kenneth W. Hance, Sara Peleg, Julia Barsony, James C. Fleet, Nucleo-cytoplasmic cycling of the vitamin D receptor in the enterocyte-like cell line, Caco-2 Journal of Cellular Biochemistry. ,vol. 100, pp. 617- 628 ,(2007) , 10.1002/JCB.21087
M. VENY, M. ESTELLER, E. RICART, J. M. PIQUÉ, J. PANÉS, A. SALAS, Late Crohn's disease patients present an increase in peripheral Th17 cells and cytokine production compared with early patients Alimentary Pharmacology & Therapeutics. ,vol. 31, pp. 561- 572 ,(2010) , 10.1111/J.1365-2036.2009.04209.X
M. NIESSNER, B. A. VOLK, Altered Th1/Th2 cytokine profiles in the intestinal mucosa of patients with inflammatory bowel disease as assessed by quantitative reversed transcribed polymerase chain reaction (RT‐PCR) Clinical and Experimental Immunology. ,vol. 101, pp. 428- 435 ,(2008) , 10.1111/J.1365-2249.1995.TB03130.X
Moana Tercel, Sarah P. McManaway, H. D. Sarath Liyanage, Frederik B. Pruijn, Preparation and Properties of Clickable Amino Analogues of the Duocarmycins: Factors That Affect the Efficiency of Their Fluorescent Labelling of DNA ChemMedChem. ,vol. 9, pp. 2193- 2206 ,(2014) , 10.1002/CMDC.201402169
Femke Baeke, Hannelie Korf, Lut Overbergh, Evelyne van Etten, Annemieke Verstuyf, Conny Gysemans, Chantal Mathieu, Human T lymphocytes are direct targets of 1,25-dihydroxyvitamin D3 in the immune system The Journal of Steroid Biochemistry and Molecular Biology. ,vol. 121, pp. 221- 227 ,(2010) , 10.1016/J.JSBMB.2010.03.037
Jessica R. de Bruyn, Rosanne van Heeckeren, Cyriel Y. Ponsioen, Gijs R. van den Brink, Mark Löwenberg, Albert J. Bredenoord, Gerard Frijstein, Geert R. D’Haens, Vitamin D deficiency in Crohn's disease and healthy controls: A prospective case–control study in the Netherlands Journal of Crohn's and Colitis. ,vol. 8, pp. 1267- 1273 ,(2014) , 10.1016/J.CROHNS.2014.03.004
Christian M. Veldman, Margherita T. Cantorna, Hector F. DeLuca, Expression of 1,25-Dihydroxyvitamin D3 Receptor in the Immune System☆ Archives of Biochemistry and Biophysics. ,vol. 374, pp. 334- 338 ,(2000) , 10.1006/ABBI.1999.1605
Vanessa O′Neill, Furaha Florence Asani, Tamsyn Jacki Jeffery, Donovan Sean Saccone, Liza Bornman, Vitamin D Receptor Gene Expression and Function in a South African Population: Ethnicity, Vitamin D and FokI PLoS ONE. ,vol. 8, pp. e67663- ,(2013) , 10.1371/JOURNAL.PONE.0067663
P. H. A. Bours, J. P. M. Wielders, J. R. Vermeijden, A. van de Wiel, Seasonal variation of serum 25-hydroxyvitamin D levels in adult patients with inflammatory bowel disease Osteoporosis International. ,vol. 22, pp. 2857- 2867 ,(2011) , 10.1007/S00198-010-1484-Y
Rocky Pramanik, John R Asplin, Christina Lindeman, Murray J Favus, Shaochun Bai, Fredric L Coe, None, Lipopolysaccharide negatively modulates vitamin D action by down-regulating expression of vitamin D-induced VDR in human monocytic THP-1 cells Cellular Immunology. ,vol. 232, pp. 137- 143 ,(2004) , 10.1016/J.CELLIMM.2005.03.004