Synthesis and study of the complex formation of a cationic alkyl-chain bola amino alcohol with DNA: in vitro transfection efficiency

摘要: Recent studies point out that bolaamphiphiles can be used in nonviral gene therapy. Due to their bipolar character, they may span a membrane and thus stabilize or destabilize it which could relevant for DNA transfer across biological membrane. Since there are only very few on bolaamphiphile application transfection, is difficult assess whether will bring additional advantages the class of vectors. A with hydrophobic chain 22 carbon atoms trimethylammonium hydroxyl groups at each end was synthesized (22-hydroxydocos-1-yl-N,N,N-trimethylammonium bromide). The conferred stability lowered zeta potential vesicles cationic lipid. Confocal laser scanning microscopy flow cytometry experiments have shown this transfect green fluorescent protein plasmid cells if mixed It compete standard lipids terms transfection but same time less toxic. molecules delivery results from fact confer high vector.