Efficient siRNA delivery by the cationic liposome DOTAP in human hematopoietic stem cells differentiating into dendritic cells.

摘要: RNA interference technology is an ideal strategy to elucidate the mechanisms associated with human id="x44" d="M43 650h280q194 282 -85q85 -82 85 -215q0 -173 -123 -267q-107 -83 -297 -83h-236v27q65 5 79.5 19.5t14.5 77.5v402q0 45 -6 62.5t-21.5 24t-57.5 9.5v28zM213 547v-416q0 -55 24.5 -75t81.5 -20q133 203 86t70 214q0 80 -30 143t-88 97q-66 41 -170 41 q-62 -78 -13q-13 -9 -13 -57z" id="x33" d="M285 378v-2q65 102 -54.5t37 -97.5q0 -30.5 -104.5t-74 -75t-85.5 -42t-72 -14.5q-31 -59.5 11t-40.5 23q-19 18 -16 36q1 16 23 33q13 10 24 0q58 -51 124 -51q55 88 40t33 112q0 64 -39 96.5t-88 32.5q-29 -64 -11l-6 29q77 25 118 57.5t41 84.5 q0 -26.5 69.5t-68.5 24.5q-67 -120 -79l-20 20l43 63q51 56 127 56h1q66 107 -37t41 -95q0 -42 -31 -71q-22 -23 -68 -54z" hematopoietic stem cell differentiation into dendritic cells. Simple manipulations in vitro can unequivocally yield alloreactive or tolerogenic populations, suggesting key implications of biochemical players that might emerge as therapeutic targets for cancer graft-versus-host disease. To knockdown proteins typically involved in biology of cells, we employed an siRNA delivery system based on cationic liposome DOTAP carrier. Freshly-isolated cells were transfected with siRNA cathepsin S negligible cytotoxicity and transfection rates (>60%) comparable efficiency shown by lentiviral vectors. Further, was performed during both commitment through entire 14-day process with repeated rounds had no effect per se development. Tested parallel, other commercially-available chemical reagents failed meet acceptable standards. In addition safe practical handling, a direct advantage over viral-mediated techniques transient silencing effects be dynamically appraised recovery of targeted proteins. Thus, our findings identify excellent reagent gene in resting differentiating potential applications related preclinical models.