PNA-based antisense oligonucleotides for micrornas inhibition in the absence of a transfection reagent.

摘要: MicroRNAs (miRNAs) are noncoding RNAs approximately 22 nucleotides in length that play a major role the regulation of important biological processes, including cellular development, differentiation, and apoptosis. Antisense oligonucleotides against miRNAs useful tools for studying mechanisms therapeutic targets miRNAs. Various antisense chemistries, peptide nucleic acids (PNAs), have been developed to enhance nuclease-resistance affinity specificity miRNA targets. PNAs greater DNA RNA than do natural acids, they resistant nucleases-an essential property an inhibitor will be exposed nucleases. However, main limiting factor use is their reduced penetration into cells. Recently, several cell-penetrating peptides (CPPs) investigated as means overcome limited PNAs. Here, we evaluated ability 11 CPPs transport inside cells absence transfection reagents then these inhibit Of tested, Tat-modified-conjugated PNA showed most effective effectively inhibited Our data demonstrate CPP suitable supporting PNA-mediated inhibition.