Cellular delivery of peptide nucleic acid (PNA).
作者： Uffe Koppelhus , Peter E. Nielsen
DOI: 10.1016/S0169-409X(02)00182-5
关键词:
摘要: Peptide nucleic acid (PNA) is a DNA mimic having pseudopeptide backbone that makes it extremely stable in biological fluids. PNA binds complementary RNA and with high affinity specificity. These qualities make leading agent among "third generation" antisense antigene agents. Unfortunately, fast progress the exploration of as an experimental therapeutical regulator gene expression has been hampered by poor cellular uptake PNA. However, number transfection protocols for have now established. include microinjection, electroporation, co-transfection DNA, conjugation to lipophilic moieties, peptides, etc. Here we give short introduction basic findings on cell-free vitro systems. This followed comprehensive evaluation most interesting literature concerning delivery intracellular effect Also current regards using co-factor reviewed.
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Anders Høgset, Lina Prasmickaite, Marit Hellum, Birgit Ø. Engesæter, Vibeke M. Olsen, Torunn E. Tjelle, Carl J. Wheeler, Kristian Berg, Photochemical transfection: a technology for efficient light-directed gene delivery. Somatic Cell and Molecular Genetics. ,vol. 27, pp. 97- 113 ,(2002) , 10.1023/A:1022979806314
Sonia Scarfi, Gianluca Damonte, Umberto Benatti, Maria Rita Mariani, Vincent G. Allfrey, Patricia L. Morris, Lidia C. Boffa, Dihydrotestosterone as a Selective Cellular/Nuclear Localization Vector for Anti-Gene Peptide Nucleic Acid in Prostatic Carcinoma Cells Cancer Research. ,vol. 60, pp. 2258- 2262 ,(2000)
Giovanna Cutrona, Elisabetta M. Carpaneto, Massimo Ulivi, Silvio Roncella, Olfert Landt, Manlio Ferrarini, Lidia C. Boffa, Effects in live cells of a c- myc anti-gene PNA linked to a nuclear localization signal Nature Biotechnology. ,vol. 18, pp. 300- 303 ,(2000) , 10.1038/73745
Lars J Brandén, Abdalla J Mohamed, CI Smith, None, A peptide nucleic acid–nuclear localization signal fusion that mediates nuclear transport of DNA Nature Biotechnology. ,vol. 17, pp. 784- 787 ,(1999) , 10.1038/11726
Liam Good, Satish Kumar Awasthi, Rikard Dryselius, Ola Larsson, Peter E. Nielsen, Bactericidal antisense effects of peptide-PNA conjugates Nature Biotechnology. ,vol. 19, pp. 360- 364 ,(2001) , 10.1038/86753
Roberto P. Stock, Alejandro Olvera, Ricardo Sánchez, Andrés Saralegui, Sonia Scarfì, Rosana Sanchez-Lopez, Marco A. Ramos, Lidia C. Boffa, Umberto Benatti, Alejandro Alagón, Inhibition of gene expression in Entamoeba histolytica with antisense peptide nucleic acid oligomers Nature Biotechnology. ,vol. 19, pp. 231- 234 ,(2001) , 10.1038/85671
Michael J. Gait, Peptide-mediated cellular delivery of antisense oligonucleotides and their analogues. Cellular and Molecular Life Sciences. ,vol. 60, pp. 844- 853 ,(2003) , 10.1007/S00018-003-3044-5
A. Prochiantz, G. Chassaing, A.H. Joliot, D. Derossi, The third helix of the Antennapedia homeodomain translocates through biological membranes Journal of Biological Chemistry. ,vol. 269, pp. 10444- 10450 ,(1994) , 10.1016/S0021-9258(17)34080-2
Trine Ljungstrøm, Helle Knudsen, Peter E. Nielsen, Cellular uptake of adamantyl conjugated peptide nucleic acids. Bioconjugate Chemistry. ,vol. 10, pp. 965- 972 ,(1999) , 10.1021/BC990053+
A. F. Faruqi, M. Egholm, P. M. Glazer, Peptide nucleic acid-targeted mutagenesis of a chromosomal gene in mouse cells Proceedings of the National Academy of Sciences of the United States of America. ,vol. 95, pp. 1398- 1403 ,(1998) , 10.1073/PNAS.95.4.1398