miR-125b inhibitor enhance the chemosensitivity of glioblastoma stem cells to temozolomide by targeting Bak1
作者： Jian Chen , Xingli Fu , Yi Wan , Zhimin Wang , Dongyi Jiang
DOI: 10.1007/S13277-014-1821-4
关键词:
摘要: Temozolomide (TMZ) is a promising chemotherapeutic agent for treating glioblastomas. However, resistance develops quickly with high frequency. Glioblastoma stem cells (GSCs) causing to drug therapy were considered be one of key factors. The mechanisms underlying GSCs TMZ are not fully understood. MicroRNAs (miRNAs) have emerged play important roles in tumorigenesis and resistance. Previous study showed that miR-125b was necessary fission making insensitive chemotherapy. Thus, exploring the functions action on TMZ-treated would valuable. In this study, we found up-regulated TMZ-resistant cells, inhibition which caused marked increase TMZ-induced cytotoxicity apoptosis subsequent decrease GSCs. Moreover, demonstrated pro-apoptotic Bcl-2 antagonist killer 1 (Bak1) direct target miR-125b. Down-regulation Bak1 inhibited led an increased TMZ. Restoring expression recovered sensitivity Taken together; our data strongly support role conferring through targeting expression.
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