Functional Differences of miR-125b on the Invasion of Primary Glioblastoma CD133-Negative Cells and CD133-Positive Cells
作者： Lei Shi , Yi Wan , Guan Sun , Xiaoyan Gu , Chunfa Qian
DOI: 10.1007/S12017-012-8188-8
关键词: Cell biology 、 Regulation of gene expression 、 microRNA 、 Cell 、 Transfection 、 Stem cell 、 Cell growth 、 Downregulation and upregulation 、 Biology 、 Cell culture
摘要: MicroRNAs (miRNAs) are small noncoding RNAs whose function as modulators of gene expression is crucial for the proper control cell development, differentiation, and homeostasis. The total number composition miRNAs expressed per at different stages development varies widely, same miRNA may differently development. In this prospective study, we evaluated miR-125b developmental glioblastoma cells, such primary cells corresponding stem cells. CD133 an important surface marker in We found that upregulation had no effects on invasion CD133-negative but it could inhibit CD133-positive cells; however, downregulation also promoted Further research into underlying mechanism demonstrated were associated with alteration MMPs (MMP-2 MMP-9) inhibitors (RECK TIMP3). Our results demonstrate plays essential role not Therefore, represent a novel target therapy targeting future.
springer.com
sci-hub.se 参考文章(34)
Donald E. Kuhn, Gerard J. Nuovo, Mickey M. Martin, Geraldine E. Malana, Adam P. Pleister, Jinmai Jiang, Thomas D. Schmittgen, Alvin V. Terry Jr., Katheleen Gardiner, Elizabeth Head, David S. Feldman, Terry S. Elton, Human chromosome 21-derived miRNAs are overexpressed in down syndrome brains and hearts. Biochemical and Biophysical Research Communications. ,vol. 370, pp. 473- 477 ,(2008) , 10.1016/J.BBRC.2008.03.120
Lei Shi, Junxia Zhang, Tianhong Pan, Jinfang Zhou, Weiyi Gong, Ning Liu, Zhen Fu, Yongping You, MiR-125b is critical for the suppression of human U251 glioma stem cell proliferation Brain Research. ,vol. 1312, pp. 120- 126 ,(2010) , 10.1016/J.BRAINRES.2009.11.056
Lin Cheng, Qiulian Wu, Olga A. Guryanova, Zhi Huang, Qian Huang, Jeremy N. Rich, Shideng Bao, Elevated invasive potential of glioblastoma stem cells. Biochemical and Biophysical Research Communications. ,vol. 406, pp. 643- 648 ,(2011) , 10.1016/J.BBRC.2011.02.123
Yong Sun Lee, Hak Kyun Kim, Sangmi Chung, Kwang-Soo Kim, Anindya Dutta, Depletion of Human Micro-RNA miR-125b Reveals That It Is Critical for the Proliferation of Differentiated Cells but Not for the Down-regulation of Putative Targets during Differentiation Journal of Biological Chemistry. ,vol. 280, pp. 16635- 16641 ,(2005) , 10.1074/JBC.M412247200
Angelo L. Vescovi, Rossella Galli, Brent A. Reynolds, Brain tumour stem cells Nature Reviews Cancer. ,vol. 6, pp. 425- 436 ,(2006) , 10.1038/NRC1889
Lei Shi, Zihao Cheng, Junxia Zhang, Rui Li, Peng Zhao, Zhen Fu, Yongping You, hsa-mir-181a and hsa-mir-181b function as tumor suppressors in human glioma cells. Brain Research. ,vol. 1236, pp. 185- 193 ,(2008) , 10.1016/J.BRAINRES.2008.07.085
E. Vlashi, C. Lagadec, L. Vergnes, T. Matsutani, K. Masui, M. Poulou, R. Popescu, L. Della Donna, P. Evers, C. Dekmezian, K. Reue, H. Christofk, P. S. Mischel, F. Pajonk, Metabolic state of glioma stem cells and nontumorigenic cells Proceedings of the National Academy of Sciences of the United States of America. ,vol. 108, pp. 16062- 16067 ,(2011) , 10.1073/PNAS.1106704108
Sheila K. Singh, Cynthia Hawkins, Ian D. Clarke, Jeremy A. Squire, Jane Bayani, Takuichiro Hide, R. Mark Henkelman, Michael D. Cusimano, Peter B. Dirks, Identification of human brain tumour initiating cells Nature. ,vol. 432, pp. 396- 401 ,(2004) , 10.1038/NATURE03128
Roger Stupp, Monika E Hegi, Targeting brain-tumor stem cells. Nature Biotechnology. ,vol. 25, pp. 193- 194 ,(2007) , 10.1038/NBT0207-193
Y. Cao, J. D. Lathia, C. E. Eyler, Q. Wu, Z. Li, H. Wang, R. E. McLendon, A. B. Hjelmeland, J. N. Rich, Erythropoietin Receptor Signaling through STAT3 Is Required for Glioma Stem Cell Maintenance Genes & Cancer. ,vol. 1, pp. 50- 61 ,(2010) , 10.1177/1947601909356352