Growth of glioblastoma is inhibited by miR-133-mediated EGFR suppression.

摘要: Glioblastoma multiforme (GBM) is a severe and highly lethal brain cancer, which malignancy largely stems from its growing in relatively restrained area the brain. Hence, understanding of molecular regulation growth GBM critical for improving treatment. Dysregulation microRNAs (miRNAs) has recently been shown to contribute development GBM, whereas role miR-133 unknown. Here, by qualitative reverse transcription polymerase chain reaction (RT-qPCR), we detected lower levels tissues, compared paired normal tissue. We overexpressed or inhibited cells. Cell apoptosis were analyzed 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay flow cytometry, respectively. found that overexpression decreased cell increased apoptosis, while depletion apoptosis. Bioinformatic analysis was performed, showing may target 3'-untranslated region (3'-UTR) epidermal factor receptor (EGFR) transduces signals. Further, protein translation inhibition EGFR confirmed dual luciferase reporter assay. Together, these data suggest reduced tissues promotes decreases possibly through targeting mRNA suppress translation.