Physiological Characteristics and Comparative Secretome Analysis of Morchella importuna Grown on Glucose, Rice Straw, Sawdust, Wheat Grain, and MIX Substrates

摘要: Morels (Morchella sp.) are an economically important edible macro-fungi, which can grow on various synthetic or semi-synthetic media. However, the complex nutritional metabolism and requirements remain ill-defined. This study, based plant biomass commonly used in morels cultivation, assessed compared growth characteristics extracellular enzymes of M. importuna cultivated glucose, rice straw, sawdust, wheat grain, a mixture equal proportions three latter substrates. could all five tested media but displayed significant variations mycelial rate, sclerotium yields different The most suitable medium for was wheat-rich medium, followed by while straw sawdust were least suitable. A total 268 secretory proteins identified LC-MS/MS detection. Functional classification label-free comparative analysis these revealed that carbohydrate-active enzyme (CAZYme) predominant component, protease, peptidase other proteins. Abundances CAZYme differed among media, ranging from 64% glucose to 88% straw. classes glycoside hydrolases carbohydrate-binding module enriched secretomes. Furthermore, activities CMCase, lignase, amylase, xylase, pNPCase pNPGase relative expression corresponding genes detected during continuous culture MIX medium. comprehensive data inferred had weak ability degrade lignocellulose strong starch. Specifically, terms degradation cellulose, cellulose into oligosaccharides much stronger with further monosaccharides, this might be speed-limiting step utilization importuna. In contrast, decompose hemicellulose glycosidic bonds, especially α- β-galactosidase. Only very few lignin-degradation-related low abundance detected, indicated lignin ability. presence lipase chitinase implied capable decomposition its own mycelia vitro. study provides key facilitates understanding