Morphological and ultrastructural examination of senescence in Morchella elata
作者： Peixin He , Yingli Cai , Sumeng Liu , Li Han , Lina Huang
DOI: 10.1016/J.MICRON.2015.07.010
关键词:
摘要: In recent years, the artificial cultivation of Morchella mushrooms that belong to Elata Clade, including elata, has been developed rapidly in China. However, prominent problem spawn aging frustrating morel farming. this paper, M. elata was achieved from 12 17 subcultures and lifespan 1536-2256 h by successive subculturing. The can be roughly divided into juvenile phase senescent with respect mycelia linear growth rate. After a certain period rapid almost constant rate at phase, isolate entered characterized slow down growth, producing pigments ahead time final death apical hyphae. definitely 240-288 h; while diverse relying on different isolates. Moreover, microscopic study showed angles between leading primary hyphae increased constantly aging. senesced hyphal cells typical characteristics autophagy (enlargement vacuoles existence organelles sequestrated lysosomes) apoptosis (condensation cytoplasm nuclear plasmolysis) were observed. addition, stage senescence, collapsed plasma membrane all cellular disrupted, indicated necrotic mode cell death. Taken together, these data revealed involvement autophagy, necrosis senescence elata. characterization molecular mechanism need further systematic will beneficial for healthy development
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