Successive subculturing alters spore-bound Pr1 activity, germination and virulence of the entomopathogenic fungus, Beauveria bassiana

作者: Seyed Ali Safavi

DOI: 10.1080/09583157.2011.588317

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摘要: One of the hurdles in development entomopathogenic fungi such as Beauveria bassiana is loss virulence when successively maintained vitro. This may result products inferior quality mass production programs. Also, there are many contradicting data and unclear points this case. Three isolates B. were subcultured 15 times. Spore-bound Pr1 activity, germination rate, conidia against mealworm (Tenebrio molitor L.) larvae studied. Results showed that normally retained their during 10 subculturings. However, they clearly offered decreased (elevated LT50 values lower percent mortality). The activity bound to declined subculturing continued; lowest spore-bound potential was recorded for 15th subculture. Virulence agreement with rate a positive correlation between P...

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