Autophagy is induced during cell death by incompatibility and is essential for differentiation in the filamentous fungus Podospora anserina
作者： Mathieu Paoletti , Karine Dementhon , Bénédicte Coulary-Salin , Corinne Clavé , Bérangère Pinan-Lucarré
DOI: 10.1046/J.1365-2958.2003.03208.X
关键词:
摘要: In filamentous fungi, a cell death reaction occurs when cells of unlike genotype fuse. This reaction, known as incompatibility is genetically controlled by set loci termed het (for heterokaryon loci). Podospora anserina, genes induced during this (idi genes) have been identified. The idi-6/pspA gene encodes serine protease that the orthologue vacuolar B Saccharomyces cerevisiae involved in autophagy. We report here PSPA participates degradative autophagic pathway Podospora. identified AUT7 S. early steps autophagy yeast. development and was designated idi-7. used GFP-IDI7 fusion protein cytological marker induction Relocalization detection bodies inside vacuoles provide evidence reaction. Therefore, fungi appears to be related type II programmed metazoans. addition, we found pspA idi-7 null mutations confer differentiation defects such absence female reproductive structures, indicating required for
sci-hub.se 参考文章(59)
Michel Castroviejo, Joël Bégueret, Corinne Clavé, Mathieu Paoletti, Identification and characterization of a gene encoding a subtilisin-like serine protease induced during the vegetative incompatibility reaction in Podospora anserina. Current Genetics. ,vol. 39, pp. 244- 252 ,(2001) , 10.1007/S002940100208
Béatrice Turcq, Joel Bégueret, The ura5 gene of the filamentous fungus Podospora anserina: nucleotide sequence and expression in transformed strains Gene. ,vol. 53, pp. 201- 209 ,(1987) , 10.1016/0378-1119(87)90008-4
J. Labarere, J. Bernet, A Mutation Inhibiting Protoplasmic Incompatibility in Podospora anserina that Suppresses an Extracellular Laccase and Protoperithecium Formation Microbiology. ,vol. 109, pp. 187- 189 ,(1978) , 10.1099/00221287-109-1-187
Eric Lam, Naohiro Kato, Michael Lawton, Programmed cell death, mitochondria and the plant hypersensitive response Nature. ,vol. 411, pp. 848- 853 ,(2001) , 10.1038/35081184
Suzana Dos Reis, Bénédicte Coulary-Salin, Vincent Forge, Ioan Lascu, Joël Bégueret, Sven J. Saupe, The HET-s Prion Protein of the Filamentous Fungus Podospora anserina Aggregates in Vitro into Amyloid-like Fibrils Journal of Biological Chemistry. ,vol. 277, pp. 5703- 5706 ,(2002) , 10.1074/JBC.M110183200
Miki Tsukada, Yoshinori Ohsumi, Isolation and characterization of autophagy-defective mutants of Saccharomyces cerevisiae. FEBS Letters. ,vol. 333, pp. 169- 174 ,(1993) , 10.1016/0014-5793(93)80398-E
Peter J. Punt, Maria A. Dingemanse, Brigit J.M. Jacobs-Meijsing, Peter H. Pouwels, Cees A.M.J.J. van den Hondel, Isolation and characterization of the glyceraldehyde-3-phosphate dehydrogenase gene of Aspergillus nidulans Gene. ,vol. 69, pp. 49- 57 ,(1988) , 10.1016/0378-1119(88)90377-0
Takayoshi Kirisako, Misuzu Baba, Naotada Ishihara, Kouichi Miyazawa, Mariko Ohsumi, Tamotsu Yoshimori, Takeshi Noda, Yoshinori Ohsumi, Formation process of autophagosome is traced with Apg8/Aut7p in yeast. Journal of Cell Biology. ,vol. 147, pp. 435- 446 ,(1999) , 10.1083/JCB.147.2.435
J. BEGUERET, Protoplasmic Incompatibility: Possible Involvement of Proteolytic Enzymes Nature. ,vol. 235, pp. 56- 58 ,(1972) , 10.1038/NEWBIO235056A0
Hélian Boucherie, Jean Bernet, Protoplasmic incompatibility and female organ formation in Podospora anserina: Properties of mutations abolishing both processes Molecular Genetics and Genomics. ,vol. 135, pp. 163- 174 ,(1974) , 10.1007/BF00264783