Flagellar motility contributes to the invasion and survival of Aeromonas hydrophila in Anguilla japonica macrophages.

摘要: The interaction between pathogenic bacteria and the host phagocytes is complicated. It generally believed that only obligate intracellular pathogens can invade survive in phagocytes. In this study, we revealed Aeromonas hydrophila B11 also macrophages of its Anguilla japonica vitro. To further investigate mechanisms A. invasion survival macrophages, a mini-Tn10 transposon mutagenesis system was used to generate an insertion mutant library by cell conjugation donor Escherichia coli Sm10 (pLOFKm) recipient B11. Out 465 individual colonies, 13 mutants impaired within were selected, BM116 most seriously strain. Molecular analysis showed ORF approximately 1335 bp (GenBank accession numbers JQ974982) inserted mini-Tn10. This putatively encodes deduced 445 amino acids protein displays highest identity (99.6%) with flagellar hook FlgE subsp. ATCC 7966. biological characteristics wild-type B11, B116 complemented strain investigated. results reveal flagella absent these exhibited defective motility, adhesion, when compared wild type These findings indicate flgE required for flagellum biogenesis motility host. Our provide important new understanding nonintracellular interactions their