Identification of the nerve terminal targets of botulinum neurotoxin serotypes A, D, and E.
作者： G Schiavo , O Rossetto , S Catsicas , P Polverino de Laureto , B.R. DasGupta
DOI: 10.1016/S0021-9258(20)80452-9
关键词:
摘要: Botulinum neurotoxins are metalloproteins with one zinc atom bound to the binding motif of endopeptidases. Here we show that botulinum neurotoxin serotypes A, D, and E endoproteases specific for components synaptic vesicle docking fusion complex. Serotypes A cleave SNAP-25, a 25-kDa protein terminal, while serotype D is VAMP/synaptobrevin, membrane vesicles. Both rat brain VAMP isoforms cleaved at single Lys-Leu peptide bond. The proteolytic activity these inhibited by EDTA captopril.
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