Quantifying cellular alignment on anisotropic biomaterial platforms.

摘要: How do we quantify cellular alignment? Cellular alignment is an important technique used to study and promote tissue regeneration in vitro vivo. Indeed, regenerative outcomes are often strongly correlated with the efficacy of alignment, making quantitative, automated assessment goal for field engineering. There currently exist various classes algorithms, which effectively address problem quantifying individual alignments using Fourier methods, kernel elliptical approximation; however, these algorithms yield population distributions limited by their inability a scalar metric alignment. The current work builds on adapting signal processing methods previously our group processes. We use automated, ellipse-fitting algorithm approximate cell body respect silk biomaterial scaffold, followed application normalized cumulative periodogram criterion produce value proposed offers generalized method assessing complex, two-dimensional environments. This may also offer novel alternative types polarity, such as fibroblasts, endothelial cells, mesenchymal stem well nuclei.