Analyzing neurite outgrowth from explants by fitting ellipses.
作者： Carmen Haines , Geoffrey J. Goodhill
DOI: 10.1016/J.JNEUMETH.2009.12.010
关键词: Neurite 、 Explant culture 、 Developing nervous system 、 Neuroscience 、 Neural development 、 Axon guidance 、 Ellipse 、 Dorsal root ganglion 、 Biology
摘要: The establishment of appropriate connectivity in the developing nervous system depends on many molecular guidance cues. A key method for studying response nerve fibers to such cues is culture explants neural tissue three-dimensional collagen gels. However, most previous analyses neurite outgrowth patterns from these have been very simple, often measuring only one or two parameters. Here we introduce a more sophisticated characterizing explants, based fitting an ellipse pattern outgrowth. This provides 5 parameters describing outgrowth: x and y position center ellipse, elongation, area tilt. We then apply this large dataset dorsal root ganglion grown presence precisely controlled gradients growth factor. analysis reveals number new features data. For instance, find that it rather than shape correlated with strength gradient. Together results show ellipse-fitting explant data can give insights into biological processes underlying by
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