Lorist6, a cosmid vector with BamHI, NotI, ScaI and Hind III cloning sites and altered neomycin phosphotransferase gene expression
作者： Toby J. Gibson , André Rosenthal , Robert H. Waterston
DOI: 10.1016/0378-1119(87)90017-5
关键词:
摘要: Four new features have been included in a phage-lambda-replicon-based cosmid vector, Lorist6. A ScaI restriction site allows insertion of blunt-ended DNA, partially digested with AluI or any other enzyme which creates blunt ends after cleavage. NotI and BamHI sites enable construction libraries using double digestion + Sau3AI. The promoter the neo gene has substituted by derived from tet pBR322, increasing expression resistance product. transcriptional terminator placed downstream to prevent transcription into insert DNAs.
sci-hub.se 参考文章(11)
GF Carle, M Frank, MV Olson, Electrophoretic separations of large DNA molecules by periodic inversion of the electric field Science. ,vol. 232, pp. 65- 68 ,(1986) , 10.1126/SCIENCE.3952500
Brigitte Cami, Philippe Kourilsky, Screening of cloned recombinant DNA in bacteria by in situ colony hybridization Nucleic Acids Research. ,vol. 5, pp. 2381- 2390 ,(1978) , 10.1093/NAR/5.7.2381
P. F. Little, S. H. Cross, A cosmid vector that facilitates restriction enzyme mapping. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 82, pp. 3159- 3163 ,(1985) , 10.1073/PNAS.82.10.3159
Brian Seed, Richard C. Parker, Norman Davidson, Representation of DNA sequences in recombinant DNA libraries prepared by restriction enzyme partial digestion Gene. ,vol. 19, pp. 201- 209 ,(1982) , 10.1016/0378-1119(82)90007-5
Annemarie Poustka, Hans Lehrach, Jumping libraries and linking libraries: the next generation of molecular tools in mammalian genetics Trends in Genetics. ,vol. 2, pp. 174- 179 ,(1986) , 10.1016/0168-9525(86)90219-2
E. Beck, G. Ludwig, E.A. Auerswald, B. Reiss, H. Schaller, Nucleotide sequence and exact localization of the neomycin phosphotransferase gene from transposon Tn5 Gene. ,vol. 19, pp. 327- 336 ,(1982) , 10.1016/0378-1119(82)90023-3
Diane K. Hawley, William R. McClure, Compilation and analysis of Escherichia coli promoter DNA sequences. Nucleic Acids Research. ,vol. 11, pp. 2237- 2255 ,(1983) , 10.1093/NAR/11.8.2237
Toby J. Gibson, Alan R. Coulson, John E. Sulston, Peter F.R. Little, Lorist2, a cosmid with transcriptional terminators insulating vector genes from interference by promoters within the insert: effect on DNA yield and cloned insert frequency Gene. ,vol. 53, pp. 275- 281 ,(1987) , 10.1016/0378-1119(87)90016-3
S.H. Cross, P.F.R. Little, A cosmid vector for systematic chromosome walking. Gene. ,vol. 49, pp. 9- 22 ,(1986) , 10.1016/0378-1119(86)90381-1
Francisco Bolivar, Raymond L. Rodriguez, Patricia J. Greene, Mary C. Betlach, Herbert L. Heyneker, Herbert W. Boyer, Jorge H. Crosa, Stanley Falkow, Construction and characterization of new cloning vehicles. II. A multipurpose cloning system. Gene. ,vol. 2, pp. 95- 113 ,(1977) , 10.1016/0378-1119(77)90000-2