Detection of Borrelia burgdorferi sensu lato in ticks: immunofluorescence assay versus polymerase chain reaction.

摘要: Immunofluorescence (IFA) and polymerase chain reaction (PCR) were examined as methods for detecting Borrelia burgdorferi sensu lato spirochaetes in unfed Ixodes ricinus nymphs. Although similar results produced some cases, a great deal of variation occurred. Furthermore, both the highly controlled initial laboratory study, involving 252 shared samples, study on field-collected ticks (n = 460), IFA tended to detect more infected than PCR. The basis these findings are yet undetermined. development quality assurance scheme is recommended so that laboratories can validate their preliminary feasibility suggested such practical.