25-Hydroxycholesterol Inhibits Adipogenic Differentiation of C3H10T1/2 Pluripotent Stromal Cells
作者： Dorothy Moseti , Alemu Regassa , Chongxiao Chen , Karmin O , Woo Kyun Kim
DOI: 10.3390/IJMS21020412
关键词:
摘要: Understanding of adipogenesis is important to find remedies for obesity and related disorders. In addition, it also critical in bone disorders because there a reciprocal relationship between osteogenesis micro-environment. Oxysterols are pro-osteogenic anti-adipogenic molecules via hedgehog activation pluripotent marrow stomal cells. However, no study has evaluated the role specific oxysterols C3H10T1/2 cells, which good cell model studying bone-marrows. Thus, we investigated effects on expression adipogenic transcripts Treatment cells with DMITro significantly induced mRNA Pparγ. This induction was inhibited by 25-HC. The C/cepα, Fabp4 Lpl To determine mechanism 25-HC inhibits adipogenesis, signalling pathway inhibitor, cyclopamine CUR61414, were evaluated. + or CUR61414 96h did not modulate adipocyte differentiation; reverse inhibitory 25-HC, suggesting that canonical may play LXR agonist inhibit but strongly Our observations showed most potent oxysterol inhibiting key among tested oxysterols, its potential application providing an intervention osteoporosis obesity. We report differentiation mediated signaling LXR.
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