Anti-arthritic Action Mechanisms of Natural Chondroitin Sulfate in Human Articular Chondrocytes and Synovial Fibroblasts
作者： Keisuke Imada , Hideki Oka , Daisuke Kawasaki , Naoyoshi Miura , Takashi Sato
DOI: 10.1248/BPB.33.410
关键词:
摘要: To clarify the exact anti-arthritic action mechanisms of chondroitin sulfate (CS), we evaluated effects CS derived from shark cartilage (CS-SC) composed mainly chondroitin-6-sulfate and porcine trachea (CS-PC) mostly chondrotin-4-sulfate on functions human articular chondrocytes synovial fibroblasts. Both CS-SC CS-PC (from 1 to 100 μg/ml) effectively suppressed interleukin (IL)-1β (10 ng/ml)-enhanced gene expression aggrecanase-1/a disintegrin metalloproteinase with thrombospondin-like motifs (ADAMTS)-4 aggrecanase-2/ADAMTS-5 in embedded alginate beads In addition, overcame IL-1β-mediated suppression aggrecan core protein mRNA, IL-1β-enhanced collagenase-3/matrix (MMP)-13 chondrocytes. CS-PC, but not recovered IL-1β-reduced tissue inhibitor metalloproteinases (TIMP)-3 chondrocytes, enhanced production TIMP-1 It is noteworthy that able modulate function fibroblasts as well Therefore, very likely be multifunctional chondroprotective material for degenerative arthritic diseases.
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