Organelle degradation in the lens by PLAAT phospholipases.

摘要: The eye lens of vertebrates is composed fibre cells in which all membrane-bound organelles undergo degradation during terminal differentiation to form an organelle-free zone1. mechanism that underlies this large-scale organelle remains largely unknown, although it has previously been shown be independent macroautophagy2,3. Here we report phospholipases the PLAAT (phospholipase A/acyltransferase, also known as HRASLS) family—Plaat1 (also Hrasls) zebrafish and PLAAT3 HRASLS3, PLA2G16, H-rev107 or AdPLA) mice4–6—are essential for such mitochondria, endoplasmic reticulum lysosomes. Plaat1 translocate from cytosol various immediately before degradation, a process requires their C-terminal transmembrane domain. translocation depends on damage membranes, both are mediated by Hsf4. After phospholipase induces extensive rupture followed complete degradation. Organelle PLAAT-family achieving optimal transparency refractive function lens. These findings expand our understanding intracellular provide insights into acquired transparent lenses. In mice, PLAAT3, respectively, macroautophagy-independent produces zone achieves transparency.