Genomic rearrangements and transcriptional analysis of the spliced leader‐associated retrotransposon in RNA interference‐deficient Trypanosoma brucei
作者： Kristin L. Patrick , Paula M. Luz , Jia-peng Ruan , Huafang Shi , Elisabetta Ullu
DOI: 10.1111/J.1365-2958.2007.06057.X
关键词:
摘要: The Trypanosoma brucei genome is colonized by the site-specific non-LTR retrotransposon SLACS, or spliced leader-associated conserved sequence, which integrates exclusively into leader (SL) RNA genes. Although there evidence that interference (RNAi) machinery regulates SLACS transcript levels, we do not know whether RNAi deficiency affects genomic stability of nor understand mechanism transcription. Here, report prolonged culturing RNAi-deficient T. cells, but wild-type results in rearrangements SLACS. Furthermore, two populations transcripts persist cells: a full-length approximately 7 kb and heterogeneous population small ranging size from 450 to 550 nt. We provide transcription initiates at +1 interrupted SL gene proceeds 5' UTR open reading frame 1 (ORF1). This carried out an polymerase with alpha-amanitin sensitivity reminiscent synthesis dependent on promoter. Additionally, show both sense antisense originate ORF1 they are associated proteins vivo. speculate serve as substrates for production siRNAs regulate expression.
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