The non-canonical CTD of RNAP-II is essential for productive RNA synthesis in Trypanosoma brucei.
作者： Anish Das , Vivian Bellofatto
DOI: 10.1371/JOURNAL.PONE.0006959
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摘要: The carboxy-terminal domain (CTD) of the largest subunit (RPB1) RNA polymerase II (RNAP-II) is essential for gene expression in metazoa and yeast. canonical CTD characterized by heptapeptide repeats. Differential phosphorylation orchestrates transcriptional co-transcriptional maturation mRNA snRNA. Many organisms, including trypanosomes, lack a CTD. In these called non-canonical or pseudo-CTD (ΨCTD. African trypanosome, Trypanosoma brucei, ΨCTD ∼285 amino acids long, rich serines prolines, phosphorylated. We report that T. brucei RNAP-II lacking entire containing only 95-amino-acid-long failed to support cell viability. contrast, with 186-amino-acid-long maintained cellular growth. truncations resulted abortive initiation transcription. These data establish CTDs play an important role expression.
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