A Nested-Polymerase Chain Reaction Protocol for Detection and Population Biology Studies of Peronospora arborescens, the Downy Mildew Pathogen of Opium Poppy, Using Herbarium Specimens and Asymptomatic, Fresh Plant Tissues
作者： Miguel Montes-Borrego , Francisco J. Muñoz Ledesma , Rafael M. Jiménez-Díaz , Blanca B. Landa
关键词:
摘要: Montes-Borrego, M., Munoz Ledesma, F. J., Jimenez-Diaz, R. and Landa, B. 2009. A nested-polymerase chain reaction protocol for detection population biology studies of Peronospora arborescens, the downy mildew pathogen opium poppy, using herbarium specimens asymptomatic, fresh plant tissues. Phytopathology 99:73-81. sensitive (PCR) was developed either two primer pairs that improves in planta arborescens DNA. The new represented an increase sensitivity 100- to 1,000-fold oomycete poppy tissue compared with limit single PCR same pairs. allowed amplification 5 0.5 fg DNA mixed Papaver somniferum proved useful amplifying from 96-year-old spp. demonstrate systemic infections by can occur wild as well cultivated poppy. Also, made possible seedborne commercial seed stocks Spain a high frequency, which poses threat spread. Direct sequencing purified amplicons alignment internal transcribed spacer (ITS) ribosomal (rDNA) sequence up 730-bp long when combining sequences obtained sets. Maximum parsimony analysis amplified ITS rDNA dubium, P. hybridum, rhoeas, different countries indicated first time degree host specificity may exist within populations arborescens. reported will be epidemiological biogeographical diseases unravel misclassification cristata, causal agents disease.
cabdirect.org
apsnet.org
sci-hub.se 参考文章(42)
D. E. L. Cooke, N. A. Williams, B. Williamson, J. M. Duncan, An Its-Based Phylogenetic Analysis of the Relationships Between Peronospora and Phytophthora Springer, Dordrecht. pp. 161- 165 ,(2002) , 10.1007/0-306-47914-1_5
Antonio Ippolito, Leonardo Schena, Franco Nigro, None, Detection of Phytophthora nicotianae and P. citrophthora in Citrus Roots and Soils by Nested PCR European Journal of Plant Pathology. ,vol. 108, pp. 855- 868 ,(2002) , 10.1023/A:1021208106857
Arne Gustavsson, The genus "Peronospora" in the Iberian peninsula, especially in northern Spain and Andorra Anales Del Jardin Botanico De Madrid. ,vol. 49, pp. 3- 38 ,(1991)
L. D. Kapoor, Opium Poppy: Botany, Chemistry, and Pharmacology ,(1995)
Jean B. Ristaino, Carol T. Groves, Gregory R. Parra, PCR amplification of the Irish potato famine pathogen from historic specimens Nature. ,vol. 411, pp. 695- 697 ,(2001) , 10.1038/35079606
M. W. Dick, Towards and Understanding of the Evolution of the Downy Mildews Springer, Dordrecht. pp. 1- 57 ,(2002) , 10.1007/0-306-47914-1_1
M. Timur Döken, The Nature of Systemic Invasion of Stem and Leaves of Sunflowers by Plasmopara helianthi Novot. var. helianthi Novot. with Mechanism of Sporulation and Zoospore Release Journal of Phytopathology. ,vol. 117, pp. 270- 275 ,(1986) , 10.1111/J.1439-0434.1986.TB00942.X
B. M. Irish, J. C. Correll, S. T. Koike, T. E. Morelock, Three New Races of the Spinach Downy Mildew Pathogen Identified by a Modified Set of Spinach Differentials. Plant Disease. ,vol. 91, pp. 1392- 1396 ,(2007) , 10.1094/PDIS-91-11-1392
Yigal Cohen, W. E. Sackston, Seed infection and latent infection of sunflowers by Plasmopara halstedii. Botany. ,vol. 52, pp. 231- 238 ,(1974) , 10.1139/B74-027
Anne P. Rehmany, James R. Lynn, Mahmut Tör, Eric B. Holub, Jim L. Beynon, A comparison of Peronospora parasitica (Downy mildew) isolates from Arabidopsis thaliana and Brassica oleracea using amplified fragment length polymorphism and internal transcribed spacer 1 sequence analyses. Fungal Genetics and Biology. ,vol. 30, pp. 95- 103 ,(2000) , 10.1006/FGBI.2000.1216