The minimum transforming region of v-abl is the segment encoding protein-tyrosine kinase.

摘要: Only 1.2 kilobases (kb) at the 5' end of 3.9-kb v-abl sequence in Abelson murine leukemia virus is required for fibroblast transformation. A precise delineation this minimum transforming region was made by using small or 3' deletions. Insertions four amino acids, generated putting synthetic DNA linkers into various restriction enzyme cleavage sites, abolished activity, indicating that much internal plays a critical role transformation process. This kb encodes protein-tyrosine kinase activity when expressed Escherichia coli. Each mutations which caused loss also resulted E. The contains acid homology to other oncogenes, and comparison with these oncogenes presented.