Lymphocyte activation. III. Binding sites for phytomitogens on lymphocyte subpopulations

摘要: The presence of binding sites for concanavalin A, phytohaemagglutinin and lentil mitogen on the surface mouse B T lymphocytes has been investigated in relation to previously described mitogenic selectivity these stimulants cells. Purified or mixed T/B suspensions cells have analysed both proportions with affinity phytomitogens relative density sites. In addition, specificity mitogens simple saccharides former cells. Microscopic agglutination immunofluorescent studies show that virtually 100% three phytomitogens. Quantitative absorption experiments demonstrated there is no difference average cells. Immunofluorescent Con-A suggested are uniformly present over entire cell surface. However, when metabolically active an interesting altered localization bound molecules one pole takes place. It various qualitatively topographically distinct but respect any quantitatively similar sites. The relevance observations lymphocyte activation considered.