Yeast 14-3-3 Protein Functions as a Comodulator of Transcription by Inhibiting Coactivator Functions

摘要: In eukaryotes combinatorial activation of transcription is an important component gene regulation. the budding yeast Saccharomyces cerevisiae, Adr1-Cat8 and Adr1-Oaf1/Pip2 are pairs activators that act together to regulate two diverse sets genes. Transcription both regulated positively by AMP-activated protein kinase homolog, Snf1, in response low glucose or presence a non-fermentable carbon source negatively redundant 14-3-3 isoforms, Bmh1 Bmh2. Bmh regulates function these at post-promoter binding step direct Adr1. However, how after activator remains unknown. present study we analyzed Bmh-mediated regulation genes activated independently activators. We report inhibits mRNA synthesis when second absent. Using fusions show Adr1 regulatory domain but not heterologous artificially recruited Mediator, consistent with acting downstream binding. assembly preinitiation complex (PIC). Gene expression studies suggest activity through coactivators Mediator Swi/Snf. recruitment appeared occur normally, PIC formation were defective, suggesting between activation.