Detection of C-Reactive Protein Utilizing Magnetic Permeability Detection Based Immunoassays

摘要: A new sensing technology platform integrating magnetic permeability detection and a two-site heterogeneous immunoassay in one-step analysis is described. As model, measurements of C-reactive protein (CRP), cardiac inflammation marker, were performed rapid (11.5 min) high-sensitivity (hs) procedure with low limit (0.2 mg/L) accuracy (CV = 11%). The was 1.2-mL disposable reagents vials containing solid phase (polyclonal anti-CRP conjugated silica microparticles), labeling agent (monoclonal superparamagnetic nanoparticles), reaction buffer. Whole blood (20 μL) assayed by introducing the sample into reagent vial using glass capillary mixing its contents hand for 30 s. After 11-min sedimentation step, placed coil detector, which measured enrichment nanoparticles solid-phase sediment. Magnetic permeabil...