Tau hyperphosphorylation in apolipoprotein E-deficient and control mice after closed head injury.

作者: L. Genis , Y. Chen , E. Shohami , D.M. Michaelson

DOI: 10.1002/(SICI)1097-4547(20000515)60:4<559::AID-JNR15>3.0.CO;2-K

关键词:

摘要: Apolipoprotein E (apoE)-deficient mice have learning and memory impairments that are associated with specific neurochemical changes hyperphosphorylation of distinct epitopes the cytoskeletal protein tau. Furthermore, such highly susceptible to sequelae brain trauma their ability recover from head injury is impaired. In present study we investigated extent neuronal maintenance repair apoE-deficient related aberrations at tau phosphorylation level. This was pursued by subjecting control closed (CHI) examination, utilizing immunoblot assays, resulting effects on phosphorylation. The results thus obtained revealed hyperphosphorylated before CHI this insult in transient hyperphosphorylation, whose time course two mouse groups varied markedly. Tau injured controls maximal about 4 hr after reverted basal levels 24 hr. contrast, almost no injury-induced observed injury. Some hyper-phosphorylation detected head-injured longer intervals, but its markedly lower than values controls. These findings show chronic brought apoE deficiency acute response both same domain mount

参考文章(23)
M Mawal-Dewan, J Henley, A Van de Voorde, J Q Trojanowski, V M Lee, The phosphorylation state of tau in the developing rat brain is regulated by phosphoprotein phosphatases. Journal of Biological Chemistry. ,vol. 269, pp. 30981- 30987 ,(1994) , 10.1016/S0021-9258(18)47378-4
M Goedert, R Jakes, R A Crowther, P Cohen, E Vanmechelen, M Vandermeeren, P Cras, Epitope mapping of monoclonal antibodies to the paired helical filaments of Alzheimer's disease : identification of phosphorylation sites in tau-protein Biochemical Journal. ,vol. 301, pp. 871- 877 ,(1994) , 10.1042/BJ3010871
K.S. Kosik, L.D. Orecchio, L. Binder, J.Q. Trojanowski, V.M.-Y. Lee, G. Lee, Epitopes that span the tau molecule are shared with paired helical filaments Neuron. ,vol. 1, pp. 817- 825 ,(1988) , 10.1016/0896-6273(88)90129-8
Gregory T. Bramblett, Michel Goedert, Ross Jakes, Sandra E. Merrick, John Q. Trojanowski, Virginia M.Y. Lee, Abnormal tau phosphorylation at Ser396 in Alzheimer's disease recapitulates development and contributes to reduced microtubule binding. Neuron. ,vol. 10, pp. 1089- 1099 ,(1993) , 10.1016/0896-6273(93)90057-X
Michel Goedert, Masato Hasegawa, Ross Jakes, Sean Lawler, Ana Cuenda, Philip Cohen, Phosphorylation of microtubule-associated protein tau by stress-activated protein kinases FEBS Letters. ,vol. 409, pp. 57- 62 ,(1997) , 10.1016/S0014-5793(97)00483-3
Shira Chapman, Daniel M. Michaelson, Specific neurochemical derangements of brain projecting neurons in apolipoprotein E-deficient mice. Journal of Neurochemistry. ,vol. 70, pp. 708- 714 ,(2002) , 10.1046/J.1471-4159.1998.70020708.X
Jean-Pierre Brion, Anne-Marie Couck, Janice Robertson, Thérèse L. F. Loviny, Brian H. Anderton, Neurofilament monoclonal antibodies RT97 and 8D8 recognize different modified epitopes in paired helical filament-tau in Alzheimer's disease Journal of Neurochemistry. ,vol. 60, pp. 1372- 1382 ,(1993) , 10.1111/J.1471-4159.1993.TB03298.X
L. Lomnitski, S. Chapman, A. Hochman, R. Kohen, E. Shohami, Y. Chen, V. Trembovler, D.M. Michaelson, Antioxidant mechanisms in apolipoprotein E deficient mice prior to and following closed head injury. Biochimica et Biophysica Acta. ,vol. 1453, pp. 359- 368 ,(1999) , 10.1016/S0925-4439(99)00010-1
Irit Gordon, Ettie Grauer, Idit Genis, Ephraim Sehayek, Daniel M. Michaelson, Memory deficits and cholinergic impairments in apolipoprotein E-deficient mice. Neuroscience Letters. ,vol. 199, pp. 1- 4 ,(1995) , 10.1016/0304-3940(95)12006-P