Single-dose protection against Plasmodium berghei by a simian adenovirus vector using a human cytomegalovirus promoter containing intron A.

摘要: Human adenovirus serotype 5 (AdH5) vector vaccines elicit strong immune responses to the encoded antigen and have been used in various disease models. We designed AdH5 vectors expressing under control of a human cytomegalovirus (HCMV) immediate-early promoter containing its intron A sequence. The transcriptional levels for with HCMV sequence (LP) were greater than those using without (SP). compared an E1E3-deleted adenoviral vector, which affords more space insertion foreign sequences, showed it be as immunogenic E1-deleted vector. Neutralizing antibodies limit efficacy based on serotype, simian offer attractive option overcome this problem. constructed encoding pre-erythrocytic-stage malarial Plasmodium berghei circumsporozoite protein. immunogenicity AdC6, recombinant 6 murine malaria model poxviral MVA FP9. AdC6 induced sterile protection from single dose 90% mice, contrast (25%) FP9 (0%). Adenoviral maintained potent CD8(+) T-cell longer period after immunization did mainly effector memory phenotype cells. Significantly, was able maintain presence preexisting immunity AdH5.