Broad neutralization by a combination of antibodies recognizing the CD4 binding site and a new conformational epitope on the HIV-1 envelope protein

摘要: Two to three years after infection, a fraction of HIV-1–infected individuals develop serologic activity that neutralizes most viral isolates. Broadly neutralizing antibodies recognize the HIV-1 envelope protein have been isolated from these patients by single-cell sorting and neutralization screens. Here, we report new method for anti–HIV-1 antibody isolation based on capturing single B cells expressed surface transfected cells. Although far less efficient than soluble baits, cell-based capture identified bind broadly epitope in vicinity V3 loop CD4-induced site (CD4i). The is cell form spike, but not forms same protein. Moreover, complement spectrum potent anti-CD4 binding (CD4bs) obtained individual. Thus, combinations with complementary can account breadth potency naturally arising activity. Therefore, vaccines aimed at eliciting should be limited epitopes.