β-(1 → 3,1 → 6)-d-glucans produced by Diaporthe sp. endophytes: Purification, chemical characterization and antiproliferative activity against MCF-7 and HepG2-C3A cells
作者： Ravely Casarotti Orlandelli , Maria de Lourdes Corradi da Silva , Ana Flora Dalberto Vasconcelos , Igor Vivian Almeida , Veronica Elisa Pimenta Vicentini
DOI: 10.1016/J.IJBIOMAC.2016.10.048
关键词:
摘要: This study reports the characterization and antiproliferative activity of exopolysaccharides (EPS) produced by submerged cultures endophytes Diaporthe sp. JF766998 JF767007 isolated from medicinal plant Piper hispidum Sw. Both strains secreted a crude EPS that, upon size exclusion chromatography, showed to contain heteropolysaccharide (galactose, glucose mannose) high-molecular weight glucan. Data methylation analysis, FTIR NMR spectroscopy (1H, COSY, TOCSY HSQC-DEPT) indicated that purified glucan consisted main chain glucopyranosyl β-(1→3) linkages substituted at O-6 glucosyl residues. According MTT assay, some treatments both β-glucans have against human breast carcinoma (MCF-7) hepatocellular (HepG2-C3A) cells after 24 48h treatment, exhibiting degree inhibition ratio reached highest values 400μg/mL: 58.0% (24h) 74.6% (48h) for MCF-7 cells, 61.0% 83.3% HepG2-C3A cells. These results represent first on effect species also expand knowledge about bioactive polysaccharides endophytic sources.
sci-hub.se 参考文章(28)
Jacques Ferlay, Isabelle Soerjomataram, Rajesh Dikshit, Sultan Eser, Colin Mathers, Marise Rebelo, Donald Maxwell Parkin, David Forman, Freddie Bray, Cancer incidence and mortality worldwide: sources, methods and major patterns in GLOBOCAN 2012. International Journal of Cancer. ,vol. 136, ,(2015) , 10.1002/IJC.29210
Ravely Casarotti Orlandelli, Tiago Tognolli de Almeida, Raiani Nascimento Alberto, Julio Cesar Polonio, João Lúcio Azevedo, João Alencar Pamphile, Antifungal and proteolytic activities of endophytic fungi isolated from Piper hispidum Sw Brazilian Journal of Microbiology. ,vol. 46, pp. 359- 366 ,(2015) , 10.1590/S1517-838246220131042
Jiezhong Chen, Beta-glucans in the treatment of diabetes and associated cardiovascular risks Vascular Health and Risk Management. ,vol. 4, pp. 1265- 1272 ,(2008) , 10.2147/VHRM.S3803
WEIJIA LI, JING ZHOU, YUYIN XU, Study of the in vitro cytotoxicity testing of medical devices (Review) Biomedical Reports. ,vol. 3, pp. 617- 620 ,(2015) , 10.3892/BR.2015.481
Sunil K. Bhanja, Chanchal K. Nandan, Soumitra Mandal, Bibhas Bhunia, Tapas K. Maiti, Soumitra Mondal, Syed S. Islam, Isolation and characterization of the immunostimulating β-glucans of an edible mushroom Termitomyces robustus var. Carbohydrate Research. ,vol. 357, pp. 83- 89 ,(2012) , 10.1016/J.CARRES.2012.04.007
Michel. DuBois, K. A. Gilles, J. K. Hamilton, P. A. Rebers, Fred. Smith, Colorimetric Method for Determination of Sugars and Related Substances Analytical Chemistry. ,vol. 28, pp. 350- 356 ,(1956) , 10.1021/AC60111A017
Sabrin H. Albeituni, Jun Yan, The effects of β-glucans on dendritic cells and implications for cancer therapy. Anti-cancer Agents in Medicinal Chemistry. ,vol. 13, pp. 689- 698 ,(2013) , 10.2174/1871520611313050003
Andrea Caroline Ruthes, Fhernanda Ribeiro Smiderle, Marcello Iacomini, d-Glucans from edible mushrooms: A review on the extraction, purification and chemical characterization approaches Carbohydrate Polymers. ,vol. 117, pp. 753- 761 ,(2015) , 10.1016/J.CARBPOL.2014.10.051
G. L. Miller, Use of Dinitrosalicylic Acid Reagent for Determination of Reducing Sugar Analytical Chemistry. ,vol. 31, pp. 426- 428 ,(1959) , 10.1021/AC60147A030
Amanda do Rocio Andrade Pires, Andrea Caroline Ruthes, Silvia Maria Suter Correia Cadena, Alexandra Acco, Philip Albert James Gorin, Marcello Iacomini, Cytotoxic effect of Agaricus bisporus and Lactarius rufus β-D-glucans on HepG2 cells. International Journal of Biological Macromolecules. ,vol. 58, pp. 95- 103 ,(2013) , 10.1016/J.IJBIOMAC.2013.03.040