作者: B.W. Steadman , K.B. Moore , K.W. Spitzer , J.H.B. Bridge
DOI: 10.1109/10.1375
关键词:
摘要: The design and characteristics of a video-based device that can noninvasively measure the extent rate shortening isolated cardiac myocytes are discussed. Construction motion detector is relatively inexpensive. It easy to use because edge selection simple video images contracting cells be analyzed at experimenter's convenience. also exhibits high spatial adequate temporal resolution. physiologic application was examined by measuring overall in enzymatically dispersed adult guinea pig rabbit bathed Tyrode's solution containing 2.7 mm calcium 36 degrees C (pH/sub 0/ 7.4). When stimulated 0.5 Hz, shortened an average 8.1% (guinea pig) 9.0% (rabbit) cell length. respectively. corresponding mean maximum rates were 81.7% 97.5% length/s, used between closely spaced markers on surface. >