Expression of Stathmin, a Developmentally Controlled Cytoskeleton-Regulating Molecule, in Demyelinating Disorders

摘要: Understanding the biological relevance of reexpression developmental molecules in pathological conditions is crucial for development new therapies. In this study, we report increased expression stathmin, a developmentally regulated tubulin-binding protein, brains patients with multiple sclerosis (MS). physiological conditions, stathmin immunoreactivity was observed polysialic acid-neural cell adhesion molecule-positive migratory progenitors subventricular zone, and its progressively decreased as cells matured into oligodendrocytes (OLs). MS patients, however, levels were elevated 2',3'-cyclic nucleotide 3'-phosphodiesterase-positive OLs, 10 bioptic samples analyzed. Increased confirmed by Western blot analysis normal-appearing white matter from brains. addition, using mass spectrometry, identified main component specific myelin protein fraction consistently preparations compared controls. To test levels, primary OL transfected myc-tagged cDNA allowed to differentiate. Consistent distinct role played molecule lineage at different stages, transient transfection favored bipolar phenotype but did not affect survival. However, sustained differentiating because overexpression, resulted enhanced apoptotic susceptibility. We conclude that demyelinating disorders could have dual role. On one hand, favoring progenitors, it may promote repair. other mature OLs indicate stress possibly