Isolation and characterization of a putative endogenous benzodiazepineoid (endozepine) from bovine and human brain.
作者： M Shoyab , L E Gentry , H Marquardt , G J Todaro
DOI: 10.1016/S0021-9258(18)67188-1
关键词:
摘要: A protein termed endozepine (EP) which inhibits the binding of benzodiazepines to synaptosomal membranes (Ki approximately 5 microM) has been purified electrophoretic homogeneity from bovine and human brain using acidic ethanol/chloroform extraction, Bio-Sil TSK-250 gel permeation chromatography, reverse-phase high performance liquid chromatographies. Bovine EP are single-chain polypeptides have molecular weights 10,000. Both proteins very hydrophilic contain an abundance lysine, glutamic, aspartic residues. Antisera prepared against used develop a sensitive radioimmunoassay for detection in tissue body fluids. immunoreactivity is widely distributed mammalian tissues, fluids, various cell lines. Substantial variation concentrations observed different regions brain.
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