Induction of neural differentiation in rat C6 glioma cells with taxol

摘要: Background Glioblastoma is a common and aggressive type of primary brain tumor. Several anticancer drugs affect GBM (glioblastoma multiforme) cells on cell growth morphology. Taxol one the widely used antineoplastic against many types solid tumors, such as breast, ovarian, prostate cancers. However, effect taxol remains unclear requires further investigation. Methods Survival rate C6 glioma under different concentrations was quantified. To clarify differentiation patterns rat challenge, survived were characterized by immunocytochemical, molecular biological, biological approaches. Results After treatment, not only death but also morphological changes, including elongation, cellular processes thinning, irregular shapes, fragmented nucleation or micronuclei, occurred in cells. Neural markers NFL (for neurons), βIII-tubulin GFAP astrocytes), CNPase oligodendrocytes) detected taxol-treated Quantitative analysis suggested significant increase percentage neural differentiated The results exhibited that may trigger Increased expression after treatment suggest some could be applied to elimination malignant cancer well changing proliferation status tumor