Quantitative analysis of volatile organic compounds released and consumed by rat L6 skeletal muscle cells in vitro

摘要: Knowledge of the release volatile organic compounds (VOCs) by cells provides important information on origin VOCs in exhaled breath. Muscle are particularly important, since their volatiles during exertion an effort contributes considerably to breath concentration profiles. Presently, cultivation human skeletal muscle is encountering a number obstacles, necessitating use animal vitro studies. Rat L6 therefore commonly used as model for studying molecular mechanisms differentiation and functions, facilitate study metabolic fate endogenously produced observed skin emanations. Within this production uptake rat were investigated using gas chromatography with mass spectrometric detection, combined head-space needle trap extraction pre-concentration technique (HS-NTE-GC-MS). Seven found be produced, whereas sixteen species consumed (Wilcoxon signed-rank test, p < 0.05) being studied. The set released included two ketones (2-pentanone 2-nonanone), sulphur (dimethyl sulfide methyl 5-methyl-2-furyl sulphide), three hydrocarbons (2-methyl 1-propene, n-pentane isoprene). Of metabolized there thirteen aldehydes (2-propenal, 2-methyl 2-propenal, propanal, 2-butenal, butanal, 3-methyl n-pentanal, n-hexanal, benzaldehyde, n-octanal, n-nonanal n-decanal), esters (n-propyl propionate n-butyl acetate), one compound disulfide). possible pathways leading these proposed discussed. An analysis showed them have huge potential identification monitoring some mechanism conditions.