Nonhuman primate breath volatile organic compounds associate with developmental programming and cardio-metabolic status

摘要: Rodent and nonhuman primate studies indicate that developmental programming by reduced perinatal nutrition negatively impacts life course cardio-metabolic health. We have developed a baboon model in which we feed control mothers (CON) ad libitum while nutrient restricted are fed 70% of global pregnancy lactation. Offspring intrauterine growth (IUGR) at term. By 3.5 years IUGR baboons showed signs insulin resistance, indicating pre-diabetic phenotype, contrast to healthy CON offspring. hypothesized novel breath analysis approach would provide markers the altered state non-invasive manner. Here assess whether exhaled volatile organic compounds (VOCs) collected from this unique cohort juvenile with documented dysfunction resulting utero can be detected their signatures. Breath was male female 4.8 ± 0.2 (human equivalent ~13 years). VOCs were quantified using two-dimensional gas chromatography mass spectrometer. Two-way ANOVA, on 76 biologically relevant identified 27 (p < 0.05) abundances between groups (sex, birthweight, sex x birthweight). The included 2-pentanone, 2-octanone, 2,2,7,7-tetramethyloctane 3-methyl-1-heptene, not previously been associated disease. Unsupervised principal component these could discriminate four clusters defining males, females, IUGR. This study, is first quantifiable signatures programing for any IUGR, demonstrates translational value identify metabolites programmed Future required validate translatability findings humans.