CD1d-restricted NKT cells: an interstrain comparison.

摘要: CD1d-restricted Va14-Ja281 invariant abTCR1 (NKT) cells are well defined in the C57BL/6 mouse strain, but they remain poorly characterized non-NK1.1-expressing strains. Surrogate markers for NKT such as abTCR1CD42CD82 and DX51CD31 have been used many studies, although their effectiveness defining this lineage remains to be verified. Here, we compare among C57BL/6, NK1.1-congenic BALB/c, and nonobese diabetic mice. were identified compared using a range of approaches: NK1.1 expression, surrogate phenotypes previous labeling with CD1d/a-galactosylceramide tetramers, cytokine production. Our results demonstrate that CD4/CD8-defined subsets present all three strains, confirm mice numerical functional deficiency these cells. We also highlight hazards phenotypes, none which accurately identify cells,and one particular (DX51CD31) actually excludes Finally, our support concept expression may not an ideal marker cells, NK1.1-negative, especially within CD41 subset particularly BALB/c