Studies on cellular adhesion in tissue culture. I. The effect of serum.

作者: L. Weiss

DOI: 10.1016/0014-4827(59)90070-9

关键词:

摘要: Abstract Some effects of serum on the adhesion tissue cells to glass in culture, are described. 1. The amount required for is greater trypsin-treated than washed cells. 2. From aspect cellular adhesion, lipoprotein-rich fraction G2 may replace whole serum. 3. A method described quantitative assessment species difference and heat inactivation results discussed terms cell-membrane structure composition culture media.

参考文章(13)
William T. McQuilkin, Mary C. Fioramonti, Wilton R. Earle, Katherine K. Sanford, Virginia J. Evans, Jay C. Bryant, Studies of nutrient media for tissue cells in vitro. I. A protein-free chemically defined medium for cultivation of strain L cells. Cancer Research. ,vol. 16, pp. 77- 86 ,(1956)
J.G. Bachtold, L.P. Gebhardt, Utilization of serum proteins by normal and poliovirus infected monkey kidney cells Experimental Cell Research. ,vol. 13, pp. 432- 435 ,(1957) , 10.1016/0014-4827(57)90032-0
M. S. C. BIRBECK, E. H. MERCER, Cell membranes and morphogenesis. Nature. ,vol. 178, pp. 985- 986 ,(1956) , 10.1038/178985B0
Irving Lieberman, Peter Ove, Purification of a serum protein required by a mammaliàn cell in tissue culture. Biochimica et Biophysica Acta. ,vol. 25, pp. 449- 450 ,(1957) , 10.1016/0006-3002(57)90511-5
R. Dulbecco, Marguerite Vogt, PLAQUE FORMATION AND ISOLATION OF PURE LINES WITH POLIOMYELITIS VIRUSES Journal of Experimental Medicine. ,vol. 99, pp. 167- 182 ,(1954) , 10.1084/JEM.99.2.167
E. H. MERCER, Electron Micrographs of Cross-Sections of Protein Monolayers Nature. ,vol. 180, pp. 87- 87 ,(1957) , 10.1038/180087A0