Regulation of basal level expression of the tryptophan operon of Escherichia coli.

摘要: Abstract Mutations in prfB, encoding release factor 2 (UGA- and UAA-specific), increase transcription termination at the trp operon attenuator Escherichia coli strains grown presence of tryptophan. The prfB mutations have no effect on basal level expression which natural leader peptide stop codon UGA was replaced by either UAG or UAA. introducing into mutant containing altered regions that influence readthrough also determined. Our findings support a model for control ribosome from codon, formation alternative transcript secondary structures, position transcribing RNA polymerase regulate expression.