Evaluation of spheroid head and neck squamous cell carcinoma cell models in comparison to monolayer cultures
作者： LORENZ KADLETZ , GREGOR HEIDUSCHKA , JULIAN DOMAYER , RAINER SCHMID , ELISABETH ENZENHOFER
DOI: 10.3892/OL.2015.3487
关键词:
摘要: Two-dimensional (2D) monolayer cell culture models are the most common method used to investigate tumor cells in vitro. In few last decades, a multicellular spheroid model has gained attention due its adjacency tumors vivo. The aim of present study was immunohistochemical differences between these two systems. FaDu, CAL27 and SCC25 head neck squamous carcinoma (HNSCC) lines were seeded out spheroids. FaDu treated with increasing doses cisplatin irradiation. not theproliferation experiments, since spheroids able process reagent CCK-8 assays. Furthermore, they stained alterations following antigens: Ki-67, vascular endothelial growth factor receptor, epithelial survivin. Differences rates expression patterns detected certain HNSCC lines. proliferation showed significant divergence grown three-dimensional compared 2D model. Overall, promising reproduce aspects characteristics cells, may show different response therapeutic options.
spandidos-publications.net参考文章(52)
Rolf Bjerkvig, Ben Slotman, Helmut Acker, Hans Petter Eikesdal, Patricia Kaaijk, Per øvind Enger, Peter Sminia, Oxygenation and response to irradiation of organotypic multicellular spheroids of human glioma. Anticancer Research. ,vol. 23, pp. 1461- 1466 ,(2003)
Ulana Kotowski, Gregor Heiduschka, Markus Brunner, Cornelia Czembirek, Christina Eder-Czembirek, Rainer Schmidt, Tammer Fahim, Dietmar Thurnher, Radiosensitization of head and neck cancer cells by the phytochemical agent sulforaphane Strahlentherapie Und Onkologie. ,vol. 187, pp. 575- 580 ,(2011) , 10.1007/S00066-011-2218-6
J C Jardiller, B Desoize, D Gimonet, Cell culture as spheroids: an approach to multicellular resistance. Anticancer Research. ,vol. 18, pp. 4147- 4158 ,(1998)
M. Ingargiola, R. Runge, J.-M. Heldt, R. Freudenberg, J. Steinbach, N. Cordes, M. Baumann, J. Kotzerke, G. Brockhoff, L.A. Kunz-Schughart, Potential of a Cetuximab-based radioimmunotherapy combined with external irradiation manifests in a 3-D cell assay. International Journal of Cancer. ,vol. 135, pp. 968- 980 ,(2014) , 10.1002/IJC.28735
Divya Khaitan, Sudhir Chandna, MB Arya, BS Dwarakanath, Establishment and characterization of multicellular spheroids from a human glioma cell line; Implications for tumor therapy Journal of Translational Medicine. ,vol. 4, pp. 12- 12 ,(2006) , 10.1186/1479-5876-4-12
Theodora Voskoglou-Nomikos, Lesley Seymour, Joseph L. Pater, Clinical Predictive Value of the in Vitro Cell Line, Human Xenograft, and Mouse Allograft Preclinical Cancer Models Clinical Cancer Research. ,vol. 9, pp. 4227- 4239 ,(2003)
Amulya K Panda, Vinod Labhasetwar, Jayme L Horning, Sanjeeb K Sahoo, Sivakumar Vijayaraghavalu, Sanja Dimitrijevic, Jaspreet K Vasir, Tapan K Jain, Vinod Labhasetwar, 3-D tumor model for in vitro evaluation of anticancer drugs Molecular Pharmaceutics. ,vol. 5, pp. 849- 862 ,(2008) , 10.1021/MP800047V
Neil Gildener-Leapman, Robert L. Ferris, Julie E. Bauman, Promising systemic immunotherapies in head and neck squamous cell carcinoma. Oral Oncology. ,vol. 49, pp. 1089- 1096 ,(2013) , 10.1016/J.ORALONCOLOGY.2013.09.009
K. W. Kross, J. H. Heimdal, C. Olsnes, J. Olofsson, H. J. Aarstad, Co-culture of head and neck squamous cell carcinoma spheroids with autologous monocytes predicts prognosis. Scandinavian Journal of Immunology. ,vol. 67, pp. 392- 399 ,(2008) , 10.1111/J.1365-3083.2008.02072.X
Mira Töyli, Linda Rosberg-Kulha, Janne Capra, Jussi Vuoristo, Sinikka Eskelinen, Different responses in transformation of MDCK cells in 2D and 3D culture by v-Src as revealed by microarray techniques, RT-PCR and functional assays. Laboratory Investigation. ,vol. 90, pp. 915- 928 ,(2010) , 10.1038/LABINVEST.2010.63