Automated DNA fragments recognition and sizing through AFM image processing
作者： E. Ficarra , L. Benini , E. Macii , G. Zuccheri
关键词:
摘要: This paper presents an automated algorithm to determine DNA fragment size from atomic force microscope images and extract the molecular profiles. The sizing of fragments is a widely used procedure for investigating physical properties individual or protein-bound molecules. Several (AFM) real computer-generated were tested different pixel sizes background noises. approach minimizes processing time with respect manual semi-automated sizing. Moreover, molecule profile recognition can be perform further structural analysis. For images, root mean square error incurred by in length estimation 0.6% 7.8 nm image 0.34% 3.9 size. AFM we obtain distribution lengths standard deviation 2.3% measured average very close one, around 0.33%.
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