Isolation and characterization of phenolic compound from Allium hookeri root for potential use as antioxidant in foods

作者: Hyun-Il Jun , Hana Jang , Dalrae Ahn , Dae Keun Kim , Jae-Heon Yang

DOI: 10.1007/S10068-015-0269-7

关键词:

摘要: This study was carried out to identify phenolic compounds with antioxidant activity from Allium hookeri root (AHR). A methanol extract of AHR sequentially fractionated and separated based on solvent polarity (methylene chloride, ethyl acetate, n-butanol) using column chromatography (Sephadex LH-20 Kiesel gel 60). The investigated by assessing the 1,1-diphenyl-1-picrylhydrazyl radical scavenging (DPPH RSA), superoxide quenching (SQA), (SRSA) isolated fractions. Based DPPH RSA results yield each fraction, n-butanol fraction (8.4-85.2% 3.9%) selected for further analysis. There no significant difference between EC50 value SRSA compound 1 purified ascorbic acid. We identified mass spectrometry (MS) nuclear magnetic resonance (NMR) as ferulic acid-4-O-β-Dglucopyranoside (FAG). Thus, this is first report a FAG AHR.

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