Development of viremia and humoral and cellular parameters of immune activation after vaccination with yellow fever virus strain 17D: A model of human flavivirus infection

摘要: To monitor early and late events of immune system activation after primary secondary flavivirus infection, 17 healthy persons were vaccinated with the standard 17D vaccine virus strain yellow fever (YF). Twelve these had not received YF previously 5 been once at least 10 years before. Viremia various parameters humoral cellular followed daily for 7 days weekly thereafter. was detected by reverse transcriptase-polymerase chain reaction in all 12 first-time vaccinees beginning from second to sixth day vaccination; most tested positive between fourth day. Infectious using a plaque forming assay serum vaccinees. As first activation, neopterin β2-microglobulin markedly increased 2 6 postvaccination. In parallel viremia, circulating CD8+ T-cells significantly increased, peak levels vaccination, indicating an system. Neither viremia nor significant changes markers observed five revaccinated persons. Neutralizing antibodies directed against developed within weeks vaccination. No correlation found extent titer neutralizing antibodies. Revaccination minor transient increase High titers persisted J. Med. Virol. 56: 159–167, 1998. © 1998 Wiley-Liss, Inc.