Cell lineage analysis of a mouse tumor.

摘要: Revealing the lineage relations among cancer cells can shed light on tumor growth patterns and metastasis formation, yet cell lineages have been difficult to come by in absence of a suitable method. We previously developed method for reconstructing trees from genomic variability caused somatic mutations. Here, we apply reconstruct, first time, tree neoplastic adjacent normal obtained laser microdissection tissue sections mouse lymphoma. Analysis reconstructed reveals that initiated single founder cell, ∼5 months before diagnosis, grew physically coherent manner, average number divisions accumulated cancerous was almost twice than lung epithelial but slightly less expected figure B lymphocytes. The were also genotyped at TP53 locus, found share common mutation, which most likely present heterozygous state. Our work shows ability obtain data regarding physical appearance, precise anatomic position, genotypic profile, position may be useful investigating development, progression, interaction with microenvironment. [Cancer Res 2008;68(14):5924–31]