Characteristics of anti-T-cell antibodies in systemic lupus erythematosus: evidence for selective reactivity with normal suppressor cells defined by monoclonal antibodies.

摘要: Abstract Monoclonal antibodies have been produced to multiple human T-cell surface antigens. While some of these define antigens represented on all peripheral T cells (T3), others are restricted in their reactivity the inducer subset (T4) or reciprocal cytotoxic/suppressor (T5/T8). In present study, relationship naturally occurring anti-T-cell found patients with systemic lupus erythematosus (SLE) subsets was examined. were purified by Sephadex G-200 anti-F(ab′)2 affinity chromatography and E rosetting techniques subsequently, suppressor eliminated T4 T8 rabbit complement. Utilizing indirect immunofluorescence Fluorescence Activated Cell Sorter (FACS), it that from 10 active reacted approximately 80% suppressor-enriched (T4−)subset. contrast, they virtually unreactive inducer-enriched (T8−)subset. studies, antibody-reactive (SLE+) SLE sera residual monoclonal FACS. These studies showed T4+ population increased after elimination SLE+ whereas T5+ diminished. Moreover, functional shown when removed, there significant enhancement pokeweed mitogen (PWM)-driven immunoglobulin synthesis autologous normal B lymphocytes. addition, lysis this same resulted an increase native DNA-driven Ig lymphocytes inactive SLE. demonstrate selectively reactive as defined antibodies.